منابع مشابه
Multitarget magnetic activated cell sorter.
Magnetic selection allows high-throughput sorting of target cells based on surface markers, and it is extensively used in biotechnology for a wide range of applications from in vitro diagnostics to cell-based therapies. However, existing methods can only perform separation based on a single parameter (i.e., the presence or absence of magnetization), and therefore, the simultaneous sorting of mu...
متن کاملSimultaneous sorting of multiple bacterial targets using integrated dielectrophoretic-magnetic activated cell sorter.
The ability to rapidly and accurately sort multiple types of biological targets-such as molecules, viruses, bacteria or mammalian cells-from complex sample mixtures is an essential component for a wide range of diagnostic and therapeutic strategies. However, most current selection methods for cell separation are either limited with regard to throughput, as is the case for Fluorescence Assisted ...
متن کاملIsolation of human myoblasts with the fluorescence-activated cell sorter.
We have established procedures for the rapid and efficient purification of human myoblasts using the fluorescence-activated cell sorter. Our approach capitalizes on the specific reaction of monoclonal antibody 5.1H11 with a human muscle cell surface antigen. For each of the five samples analyzed, an enrichment of myoblasts to greater than 99% of the cell population was immediately achieved. Fol...
متن کاملEfficient immunoselection of cytolytic effectors with a magnetic cell sorter.
This paper describes a rapid and efficient method for the sorting of in vitro activated cytolytic effectors cells. For cytotoxic assays, a large number of cells with conserved function must be rapidly obtained. Immunomagnetic sorting was chosen because it is faster than flow cytometry sorting. The MACS system requires the use of paramagnetic beads of small diameter (100-150 nm), reputed to inte...
متن کاملAnalysis of Pneumocystis carinii cysts with a fluorescence-activated cell sorter.
Human sera from Pneumocystis carinii-infected patients and specific rabbit antisera have antibodies against the cyst form of the organism. Lung tissue concentrations from cortisone-treated C3H/HeN mice and six open lung biopsy-positive patients were centrifuged and suspended, and immunofluorescent staining was done. We utilized the fluorescence-activated cell sorter to analyze and sort P. carin...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2008
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0809795105